Sök ett spännande exjobb hos Affibody. Läs mer om projektet och hur man söker HÄR!
Affibody is a Swedish biotech company focused on developing next generation biopharmaceuticals based on its unique proprietary technology platforms: Affibody® molecules and Albumod® half-life extension technology.
In the research operations department, we develop and characterize new Affibody® molecules. The ones with the most interesting properties are then selected as CD (Candidate Drug). In the spring of 2026, we are planning a 30 credits degree project with the preliminary title:
Development of fluorescence labeled HSA assays for
visualization of cell-binding Affibody® molecules
Background
Affibody AB runs several early discovery projects aimed at identifying novel, clinically
relevant Affibody® molecules. Candidate molecules are subjected to a broad spectrum of
in vitro pharmacology studies to characterize their activity. A key aspect to support Affibody’s innovative radiopharmaceutical pipeline is to identify Affibody® molecules with
strong and specific binding to cellular receptor targets.
Execution
Cell binding assays are commonly used to identify molecules that can bind to tumor cell
targets. If the targeting molecule is a monoclonal antibody there are several commercially
available reagents for detection of bound antibodies. With Affibody® molecules an extra
staining step with proprietary antibody reagents are currently needed. The project aims to
develop an alternative way of visualizing cell-bound Affibody® molecules by utilizing
the strong interaction between the albumin-binding domain (ABD), often fused to Affibody® molecules for half-life extension, and human serum albumin (HSA).
Key features included in the project:
- Evaluate different fluorophores and techniques for labeling HSA.
- Perform routine cell culture in sterile environment.
- Develop and optimize cell staining protocols for different tumor in vitro cell models.
- Analyze stained cells with flow cytometry and in plate reader.
- Quality testing of final new protocol.
Optional activities: - Evaluate stained cells with fluorescence microscopy and image analysis.
Supervisor
Lotta Tengroth, PhD. If you have any questions, please contact: lotta.tengroth@affibody.se. Affibody AB, Scheeles väg 2, 171 65 Solna.
Application
If you are interested, send your personal letter and CV to jobs@affibody.se latest November 14th, 2025 and mark your application with EX049.